The effects of Sideritis akmanii on endoplasmic reticulum stress, inflammation, and DNA damage in experimentally ER-stress-induced MCF-7 cancer cells

Cancer is one of the diseases that became a social problem can happen with uncontrolled proliferation, growth, differentiation, and spread cells in our body. Breast cancer, on other hand, types cancer highest incidence women. In study, endoplasmic reticulum (ER) stress induced by thapsigargin (T) MCF-7 then, effects Sideritis akmanii acetone extract (SAE) cell viability, ER stress, inflammation, DNA damage were investigated. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test was used to determine effect SAE cytotoxicity comet (SCGE; single-cell gel electrophoresis) assay for genotoxicity. Additionally, mRNA expression levels both parameters (ATF4: activating transcription factor 4, ATF6: 6, PERK: protein kinase RNA-like kinase, GRP78: glucose-regulated 78) inflammation-related (TNF alpha: tumor necrosis factor-alpha, IFN-gamma: interferon-gamma, IL-6: interleukin-6, IL-8: interleukin-8, IL-12: interleukin-12) determined qPCR. results showed increased as result T treatment, caused decreased when low dose administered high further levels. It SAE, different doses or alone experimental groups, all inflammatory genes compared control group. As result, it has been S. akmanii, especially at doses, may exhibit anticarcinogenic through its genotoxic, cytotoxic, cells.